Follow these steps to exclude a segment from receiving broadcast email :

From you dashboard, click on Broadcast from left side bar.
Click on " + " icon to create a broadcast.

Select/check the segment you want to exclude from the include section.
Also check "All subscriber in this list" on Include Recipients section.
Click on next.

Design the email template and click next.

Click next to proceed to Confirm.
Confirm the time when you want to send this broadcast and click Send.
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